PCR Primers and Conditions


This page is under construction! Numerous primer pairs and PCR conditions have been reported in one or more papers which the Krawetz group has published. The publications in which each primer pair has been reported will be indicated in the "notes" section for each primer set. Unless otherwise noted, the primer sets presented here have been identified using Oligo, by NBI.

Contents:

Human Protamine related genes:
hP3.1A/B
hP3.1C/D
9MAR
10MAR
P1N/C
P2GA repeat
P2N/C
P2 3' UTR
hP3.1E/F
TNP2
30MAR
hP3.1G/H
hP3.1I/J
Mouse Protamine related genes:
mus PRM1
mus PRM2
mus TNP2
Primers from other haploid expressed regions:
Pgk2
mus pgk2
Acrosin
Other primers
Universal beta-Actin
beta-Globin major
mus beta-Globin major
alpha globin H2A
Pgk1
mus pgk1

Human Protamine locus primers

Amplicon ID: hP3.1A/B

Amplicon ID: hP3.1C/D

Amplicon ID: 9MAR

Amplicon ID: 10MAR

Amplicon ID: P1N/C

Amplicon ID: P2GA

Amplicon ID: P2N/C

Amplicon ID: P2 3' UTR

Amplicon ID: hP3.1E/F

Amplicon ID: TNP2 region

Amplicon ID: 30MAR

Amplicon ID: hP3.1G/H

Amplicon ID: hP3.1I/J

Mouse protamine locus primers

Amplicon ID: mouse protamine 1

Amplicon ID: mouse protamine 2

Amplicon ID: mouse transition protein 2

Other haploid expressed loci

Amplicon ID: PGK2

Amplicon ID: mPgk2

Amplicon ID: Acrosin

Other primer sets

Amplicon ID: Universal beta-Actin

Amplicon ID: beta-globin

Amplicon ID: mouse beta-globin

Amplicon ID: alpha globin H2A

Amplicon ID: PGK1

Amplicon ID: mPgk1